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KMID : 0861020130280020045
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Korea Journal of Herbology
2013 Volume.28 No. 2 p.45 ~ p.53
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Neuroprotective effects of some herbal medicine plant extract against ischemia·reperfusion-induced cell death in SK-N-SH neuronal cells
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오태우:Oh Tae-Woo
이미영:Lee Mi-Young/이혜원:Lee Hye-won/박용기:Park Yong-Kee
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Abstract
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Objectives : The purpose of the study is to determine the neuroprotective effects of the water and 80% EtOH extract
of some herbal medicine plant on ischemia·reperfusion-induced cell death in SK-N-SH human brain neuronal cells.
Methods : SK-N-SH cells were treated with 3mM sodium azide and 10 mM 2-deoxy-D-glucose for 45 min,
ptior to the addition of different concentrations of herbal medicine plant extract (0, 10, 25, 50, 100, 250, 500,
1000 μg/ml) for 2 hr and then reperfused with growth medium, incubated for 24 h. Cell viability was
determined by WST-1 assay, and ATP/ADP levels were measured by ADP/ATP ratio assay kit.
Results : Herbal medicine plant extract significantly inhibited decreasing the cell viability in ischemia-induced
SK-N-SH cells. Also increased the ratio of ADP/ATP in ischemia-induced neuronal cells.
Conclusions : Our results suggest that herbal medicine plant extract has a neuroprotective property via
increasing the energy levels in neuronal cells, suggesting that extract may has a therapeutic potential in the
treatment of ischemic brain injury. The exact component and mechanism remains for the future study.
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KEYWORD
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Herbal medicin plant, SK-N-SH neuroblastoma cell, Chemical ischemic injury, neuroprotective effect
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